Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CRALBP antibody [EPR23448-119] (ab243664)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23448-119] to CRALBP
Suitable for: IHC-P, IP, WB, IHC-Fr, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-CRALBP antibody [EPR23448-119]
See all CRALBP primary antibodies
Description

Rabbit monoclonal [EPR23448-119] to CRALBP
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human eyeball tissue lysate; Mouse eyeball tissue and P20 retina lysates; Rat eyeball tissue lysate; SK-MEL-2 and SK-MEL-28 whole cell lysates. IHC-P: Rat retina tissue, Mouse retina tissue; Human retina tissue. IHC-Fr: Mouse retina tissue; Rat retina tissue. Flow Cyt: SK-MEL-28 cells. IP: SK-MEL-28 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23448-119
Isotype

IgG
Research areas

Western blot - Anti-CRALBP antibody [EPR23448-119] (ab243664)

All lanes : Anti-CRALBP antibody [EPR23448-119] (ab243664) at 1/1000 dilution

Lane 1 : SK-MEL-2 (human skin malignant melanoma), whole cell lysate
Lane 2 : SK-MEL-28 (human malignant melanoma), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 29063836).

Exposure time: 15 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)

Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human retina. The section was incubated with ab243664 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Flow Cytometry - Anti-CRALBP antibody [EPR23448-119] (ab243664)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SK-MEL-28 (Human malignant melanoma) cells labelling CRALBP with ab243664 at 1/700 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-CRALBP antibody [EPR23448-119] (ab243664)

CRALBP was immunoprecipitated from 0.35 mg SK-MEL-28 (human malignant melanoma), whole cell lysate with ab243664 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243664 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: SK-MEL-28 (human malignant melanoma), whole cell lysate 10 ug

Lane 2: ab243664 IP in SK-MEL-28 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243664 in SK-MEL-28 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 100 seconds
Western blot - Anti-CRALBP antibody [EPR23448-119] (ab243664)

All lanes : Anti-CRALBP antibody [EPR23448-119] (ab243664) at 1/1000 dilution

Lane 1 : Mouse eyeball tissue lysate
Lane 2 : Rat eyeball tissue lysate
Lane 3 : Mouse P20 retina tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 26199951)

Exposure time: 15 seconds.
Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 peremeabilised mouse retina tissue labeling CRALBP with ab243664 at 1/250 dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on mouse retina especially on retinal pigment epithelial cell (RPE) and Müller cells. The nuclear counter stain is DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Western blot - Anti-CRALBP antibody [EPR23448-119] (ab243664)

Anti-CRALBP antibody [EPR23448-119] (ab243664) at 1/1000 dilution + Human eyeball tissue lysate at 20 µg

Secondary
VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 29063836).

Exposure time: 3.25 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)

Immunohistochemical analysis of paraffin-embedded Rat retina tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat retina. The section was incubated with ab243664 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 peremeabilised rat retina tissue labeling CRALBP with ab243664 at 1/250 dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Positive staining on rat retina especially on retinal pigment epithelial cell (RPE) and Müller cells. The nuclear counter stain is DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)

Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse retina. The section was incubated with ab243664 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [EPR23448-119] (ab243664)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CRALBP with ab243664 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on human kidney. The section was incubated with ab243664 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Anti-CRALBP antibody [EPR23448-119] (ab243664)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com