Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2133Y] to RSK1 p90 (phospho T573)
- Suitable for: ICC/IF, WB
- Reacts with: Human
Overview
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Product name
Anti-RSK1 p90 (phospho T573) antibody [EP2133Y]
See all RSK1 p90 primary antibodies -
Description
Rabbit monoclonal [EP2133Y] to RSK1 p90 (phospho T573) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562 cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2133Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) at 1/2000 dilution (purified)
Lane 1 : Untreated Ramos whole cell lysate
Lane 2 : Ramos treated with Phorbol-12-myristate-13-acetate whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Immunofluorescence staining of K562 cells with purified ab62324 at a working dilution of 1/500, counter-stained with DAPI. The cells were treated with 500 nM TPA for 15 minutes. Both the treated and untreated cells are shown. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the right hand panels. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom panels - for negative control 1, purified ab62324 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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All lanes : Anti-RSK1 p90 (phospho T573) antibody [EP2133Y] (ab62324) at 1/500 dilution (Unpurified)
Lane 1 : K562 cell lysate; untreated
Lane 2 : K562 cell lysate; treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP conjugated, at 1/2000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
Beta Tubulin has been included as a loading control. -