Anti-RSK1 p90 antibody (ab223616)
Key features and details
- Rabbit polyclonal to RSK1 p90
- Suitable for: ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RSK1 p90 antibody
See all RSK1 p90 primary antibodies -
Description
Rabbit polyclonal to RSK1 p90 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human RSK1 p90 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q15418
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), Tris buffered saline -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 antibody (ab223616)
2% formaledehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RSK1 p90 (yellow) using ab223616 at 1/50 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit R-PE at 1/200 dilution for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at room temperature.
(A) DAPI (blue) nuclear stain. (B) ab223616. (C) Merge.
Cells heat shocked at 42°C for 30 minutes.
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Immunoprecipitation - Anti-RSK1 p90 antibody (ab223616)RSK1 p90 was immunoprecipitated from mouse brain lysate with ab223616 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab223616 at 1/1000 dilution.
Lane 1: ab223616 IP in mouse brain lysate.
Lane 2: IP negative control.
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Immunocytochemistry/ Immunofluorescence - Anti-RSK1 p90 antibody (ab223616)2% formaledehyde-fixed heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RSK1 p90 (green) using ab223616 at 1/50 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit FITC at 1/200 dilution for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at room temperature.
(A) DAPI (blue) nuclear stain. (B) ab223616. (C) Merge.
Cells heat shocked at 42°C for 30 minutes.
