Anti-RSK1 p90 antibody [Y81] (ab32526)
![Anti-RSK1 p90 antibody [Y81] (ab32526)](https://www.abcam.com/ps/products/32/ab32526/Images/ab32526-257571-anti-rsk1-p90-antibody-y81-western-blot.jpg "Anti-RSK1 p90 antibody [Y81] (ab32526)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y81] to RSK1 p90
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-RSK1 p90 antibody [Y81]
See all RSK1 p90 primary antibodies -
Description
Rabbit monoclonal [Y81] to RSK1 p90 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human RSK1 p90 (C terminal). The exact sequence is proprietary.
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Positive control
- MCF7 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
Y81 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RSK1 p90 antibody [Y81] (ab32526)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: RSK1 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF-7 cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32526 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32526 was shown to recognize RSK1 when RSK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and RSK1 knockout samples were subjected to SDS-PAGE. ab32526 and ab8245 (loading control to GADPH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-RSK1 p90 antibody [Y81] (ab32526)](https://www.abcam.com/ps/products/32/ab32526/Images/ab32526-45111-anti-rsk1-p90-antibody-y81-western-blot.jpg)
Western blot - Anti-RSK1 p90 antibody [Y81] (ab32526)Anti-RSK1 p90 antibody [Y81] (ab32526) at 1/1000 dilution + MCF7 cell lysate
Predicted band size: 83 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
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![Anti-RSK1 p90 antibody [Y81] (ab32526)](https://www.abcam.com/ps/products/32/ab32526/Images/ab32526-3-benefits-of-recombinant-antibodies.png)
Anti-RSK1 p90 antibody [Y81] (ab32526)
