Anti-MEK1 + MEK2 antibody [3D9] (ab69502)
Key features and details
- Mouse monoclonal [3D9] to MEK1 + MEK2
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-MEK1 + MEK2 antibody [3D9]
See all MEK1 + MEK2 primary antibodies -
Description
Mouse monoclonal [3D9] to MEK1 + MEK2 -
Host species
Mouse -
Specificity
Recognizes human MEK1 and MEK2. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Native MEK and recombinant MEK1
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Positive control
- Brain. Jurkat cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3D9 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-MEK1 + MEK2 antibody [3D9] (ab69502) at 0.5 µg/ml
Lane 1 : Jurkat whole cell lysates at 20 µg
Lane 2 : Jurkat whole cell lysates at 40 µg
Lane 3 : recombinant MEK2 inactive at 0.02 µg
Lane 4 : recombinant MEK1 inactive at 0.02 µg
Lane 5 : recombinant MEK1 active at 0.02 µg
Lane 6 : recombinant MEK2 active at 0.02 µg
Lane 7 : MW ladder
Secondary
All lanes : HRP-conjugated goat
anti-mouse secondary antibody
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 43 or 71 kDa why is the actual band size different from the predicted?
Recombinant MEK1 (active and inactive) has a GST Tag resulting in a MW of ~71 kDa. The incubation with the primary antibody was carried o/n at 4°C. -
Formalin-fixed, paraffin-embedded brain tissue stained with 1 µg/mL anti-MEK ab69502.