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Signal Transduction Protein Phosphorylation Ser / Thr Kinases MAPK Pathway

Anti-MEK1 + MEK2 antibody [3D9] (ab69502)

Anti-MEK1 + MEK2 antibody [3D9] (ab69502)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [3D9] to MEK1 + MEK2
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-MEK1 + MEK2 antibody [3D9]
    See all MEK1 + MEK2 primary antibodies
  • Description

    Mouse monoclonal [3D9] to MEK1 + MEK2
  • Host species

    Mouse
  • Specificity

    Recognizes human MEK1 and MEK2.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Native MEK and recombinant MEK1

  • Positive control

    • Brain. Jurkat cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    3D9
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway

Images

  • Western blot - Anti-MEK1 + MEK2 antibody [3D9] (ab69502)
    Western blot - Anti-MEK1 + MEK2 antibody [3D9] (ab69502)
    All lanes : Anti-MEK1 + MEK2 antibody [3D9] (ab69502) at 0.5 µg/ml

    Lane 1 : Jurkat whole cell lysates at 20 µg
    Lane 2 : Jurkat whole cell lysates at 40 µg
    Lane 3 : recombinant MEK2 inactive at 0.02 µg
    Lane 4 : recombinant MEK1 inactive at 0.02 µg
    Lane 5 : recombinant MEK1 active at 0.02 µg
    Lane 6 : recombinant MEK2 active at 0.02 µg
    Lane 7 : MW ladder

    Secondary
    All lanes : HRP-conjugated goat
    anti-mouse secondary antibody

    Developed using the ECL technique.

    Predicted band size: 43 kDa
    Observed band size: 43 or 71 kDa
    why is the actual band size different from the predicted?



    Recombinant MEK1 (active and inactive) has a GST Tag resulting in a MW of ~71 kDa. The incubation with the primary antibody was carried o/n at 4°C.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 + MEK2 antibody [3D9] (ab69502)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 + MEK2 antibody [3D9] (ab69502)
    Formalin-fixed, paraffin-embedded brain tissue stained with 1 µg/mL anti-MEK ab69502.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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