Anti-CACNA2D2 antibody [EPR12343] (ab173293)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12343] to CACNA2D2
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-CACNA2D2 antibody [EPR12343]
See all CACNA2D2 primary antibodies -
Description
Rabbit monoclonal [EPR12343] to CACNA2D2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Recombinant fragment within Human CACNA2D2 aa 50-200. The exact sequence is proprietary.
Database link: Q9NY47 -
Positive control
- Fetal lung, fetal brain and Human cerebellum lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12343 -
Isotype
IgG -
Research areas
Images
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CACNA2D2 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab173293 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab173293 was shown to specifically react with CACNA2D2 when CACNA2D2 knockout samples were used. Wild-type and CACNA2D2 knockout samples were subjected to SDS-PAGE. ab173293 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
All lanes : Anti-CACNA2D2 antibody [EPR12343] (ab173293) at 1/1000 dilution
Lane 1 : Fetal lung lysate
Lane 2 : Fetal brain lysate
Lane 3 : Human cerebellum lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 130 kDa
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Western blot analysis on immunoprecipitation pellet from fetal brain lysate using ab173293.
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