Anti-Antithrombin III/ATIII antibody [EP5372] (ab126598)
Key features and details
- Rabbit monoclonal [EP5372] to Antithrombin III/ATIII
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Antithrombin III/ATIII antibody [EP5372]
See all Antithrombin III/ATIII primary antibodies -
Description
Rabbit monoclonal [EP5372] to Antithrombin III/ATIII -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
corresponding to Human Antithrombin III/ATIII aa 350-450.
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Positive control
- Human plasma, Human ovary cancer, Human testis and fetal lung lysates. Human liver tissue.
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General notes
Previously labelled as Antithrombin III.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.26 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP5372 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Antithrombin III/ATIII antibody [EP5372] (ab126598) at 1/1000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human ovary cancer lysate
Lane 3 : Human testis lysate
Lane 4 : Fetal lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Antithrombin III/ATIII antibody [EP5372] (ab126598)
ab126598, at 1/50 dilution, staining Antithrombin III/ATIII in paraffin-embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.