Human Antithrombin III / ATIII ELISA Kit (ab222507)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.33 ng/ml
- Range: 3.13 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Antithrombin III / ATIII ELISA Kit
See all Antithrombin III/ATIII kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.9% Inter-assay Sample n Mean SD CV% Serum 3 7.9% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.33 ng/ml -
Range
3.13 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 120 106% - 133% Milk 113 100% - 126% Urine 87 80% - 93% Serum 91 79% - 106% Cell culture media 110 97% - 122% Hep Plasma 106 97% - 121% EDTA Plasma 100 102% - 110% Cit plasma 107 79% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Antithrombin III / ATIII ELISA Kit (ab222507) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Antithrombin III / ATIII protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Antithrombin III / ATIII with 0.33 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Antithrombin-III (ATIII), also known as Serpin C1, is a 58 kDa extracellular glycoprotein belonging to the Serpin serine protease inhibitor superfamily. Antithrombin III functions primarily as a blood anticoagulant but also modulates inflammatory cytokine expression. Human Antithrombin-III is synthesized in the liver and is secreted as a 432 amino acid (aa) mature protein. Most circulating Antithrombin-III (90-95%) is the fully glycosylated a-isoform while the partially glycosylated ß-isoform constitutes 5-10%. The biological activity of Antithrombin-III is inherently low and is enhanced up to 1000-fold in the presence of heparin or heparin-like glycosaminoglycans. The ß-isoform of Antithrombin-III has a higher affinity for heparin. Mature human Antithrombin-III shares 90% sequence homology with mature mouse Antithrombin-III. Human Antithrombin-III inactivates thrombin and other blood coagulation factors. Human adult Antithrombin-III levels in plasma are relatively constant. However, reduction of Antithrombin-III is observed in individuals with diabetes or sepsis. Neonates also exhibit low levels of Serpin C1 in plasma.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Antithrombin III Capture Antibody 1 x 600µl 10X Human Antithrombin III Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent 5BC 1 x 6ml Denaturant 1 x 500µl Human Antithrombin III Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. -
Tissue specificity
Found in plasma. -
Involvement in disease
Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- ANT3_HUMAN
- Antithrombin
- Antithrombin III
see all -
Database links
- Entrez Gene: 462 Human
- Omim: 107300 Human
- SwissProt: P01008 Human
- Unigene: 75599 Human
Images
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Other - Human Antithrombin III / ATIII ELISA Kit (ab222507)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Antithrombin III standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Antithrombin III in human serum and plasma samples.The concentrations of Antithrombin III were measured in duplicates, interpolated from the Antithrombin III standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:4,000 plasma (citrate) 1:8,000, plasma (EDTA) 1:4,000, and plasma (heparin) 1:4,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Antithrombin III concentration was determined to be 157.69 µg/mL in neat serum, 279.95 µg/mL in neat plasma (citrate), 160.27 µg/mL in neat plasma (EDTA), and 224.88 µg/mL in neat plasma (heparin).
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Interpolated concentrations of native Antithrombin III in human milk and human Hep G2 supernatant samples and spiked Antithrombin III in human urineThe concentrations of Antithrombin III were measured in duplicates, interpolated from the Antithrombin III standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 3.13%, Hep G2 supernatant 6.25%, and urine 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Antithrombin III concentration was determined to be 810.16 ng/mL in neat milk, 218.86 ng/mL in neat Hep G2 supernatant, and 618.60 ng/mL in neat spiked urine.
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Serum from ten individual healthy human female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Antithrombin III concentration was determined to be 77.20 µg/mL with a range of 34.09 – 121.12 µg/mL.
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Sandwich ELISA - Human Antithrombin III / ATIII ELISA Kit (ab222507)To learn more about the advantages of recombinant antibodies see here.
