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Mouse ACE ELISA Kit (ab256400)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 24 pg/ml
  • Range: 46.88 pg/ml - 3000 pg/ml
  • Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse ACE ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 2.6%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 1%

  • Sample type

    Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    24 pg/ml

  • Range

    46.88 pg/ml - 3000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 104 98% - 119%
    Serum 83 77% - 93%
    Cell culture media 88 84% - 93%
    Hep Plasma 106 102% - 114%
    EDTA Plasma 104 90% - 122%
    Cit plasma 122 104% - 141%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow

  • Product overview

    Mouse ACE ELISA Kit (ab256400) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ACE protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse ACE with 24 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    ACE (Angiotensin-converting enzyme) converts angiotensin I to angiotensin II by release of the terminal His-Leu dipeptide, this results in an increase of the vasoconstrictor activity of angiotensin. It is also able to inactivate bradykinin, a potent vasodilator. ACE has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of mouse ACE standard curve in Sample Diluent NS.
    Example of mouse ACE standard curve in Sample Diluent NS.

    The ACE standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native ACE in mouse serum and plasma samples.
    Interpolated concentrations of native ACE in mouse serum and plasma samples.

    The concentrations of ACE were measured in duplicates, interpolated from the ACE standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400, plasma (citrate) 1:400, plasma (EDTA) 1:400 and plasma (heparin) 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACE concentration was determined to be 928 ng/mL in neat serum, 762 ng/mL in neat plasma (citrate), 763 ng/mL in neat plasma (EDTA)and 901 ng/mL in neat plasma (heparin).

  • Interpolated concentrations of native ACE in mouse kidney tissue culture supernatant sample.
    Interpolated concentrations of native ACE in mouse kidney tissue culture supernatant sample.

    The concentrations of ACE were measured in duplicates, interpolated from the ACE standard curves and corrected for sample dilution. Undiluted samples are as follows: kidney tissue culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACE concentration was determined to be 61 ng/mL in neat kidney tissue culture supernatant.

  • Sandwich ELISA - Mouse ACE ELISA Kit (ab256400)
    Sandwich ELISA - Mouse ACE ELISA Kit (ab256400)
    To learn more about the advantages of recombinant antibodies see here.

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