Human Antithrombin III ELISA Kit (ab108801)
Key features and details
- Sensitivity: 3 ng/ml
- Range: 3.13 ng/ml - 200 ng/ml
- Sample type: Cell culture media, Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Antithrombin III ELISA Kit
See all Antithrombin III/ATIII kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.8% Inter-assay Sample n Mean SD CV% Overall 7.1% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture media, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
= 3 ng/ml -
Range
3.13 ng/ml - 200 ng/ml -
Recovery
98.5 %
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Assay time
4h 0m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Antithrombin III Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Antithrombin III in urine, plasma, milk, saliva, cerebrospinal fluid, serum, and cell culture supernatants.
An antithrombin III specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an antithrombin III specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of antithrombin III captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Antithrombin III Antibody 1 x 120µl Antithrombin III Microplate (12 x 8 well strips) 1 unit Antithrombin III Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. -
Tissue specificity
Found in plasma. -
Involvement in disease
Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- ANT3_HUMAN
- Antithrombin
- Antithrombin III
see all -
Database links
- Entrez Gene: 462 Human
- Omim: 107300 Human
- SwissProt: P01008 Human
- Unigene: 75599 Human
Images
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ELISA: Antithrombin III Human ELISA Kit
Linearity: Antithrombin III in samples at different diutions, corrected for the factor of dilution (duplicates +/- SD).
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ELISA: Antithrombin III Human ELISA KitLinearity: Antithrombin III in samples at different diutions, corrected for the factor of dilution (duplicates +/- SD), showing ng x mL-1 on y-axis.
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ELISA: Antithrombin III Human ELISA KitLinearity: Antithrombin III in samples at different diutions, corrected for the factor of dilution (duplicates +/- SD).
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ELISA: Antithrombin III Human ELISA KitAntithrombin III in cell culture supernatants (duplicates +/- SD), tested in the dilution range of 1:1-1:100.
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ELISA: Antithrombin III Human ELISA KitAntithrombin III in human biological fluids (duplicates +/- SD). The samples were differently diluted (serum and Li-heparine plasma: 1e4-1e5; CSF: 1e2-1e3; milk: 10-100; saliva: 5-100; urine: neat).
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Typical Standard CurveRepresentative standard curve using ab108801.
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Typical Standard CurveRepresentative standard curve using ab108801.
