Mouse uPA Total ELISA Kit (URK) (ab198512)
Key features and details
- Sensitivity: 0.00998 ng/ml
- Range: 0.01 ng/ml - 10 ng/ml
- Sample type: Plasma
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Mouse
Overview
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Product name
Mouse uPA Total ELISA Kit (URK)
See all uPA kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Known Conc 20 0.06ng/ml 0.005 8.12% Known Conc 20 0.3ng/ml 0.019 6.18% Known Conc 20 3.01ng/ml 0.112 3.73% Inter-assay Sample n Mean SD CV% Known Conc 10 0.09ng/ml 0.008 8.52% Known Conc 10 0.25ng/ml 0.019 7.47% Known Conc 10 2.66ng/ml 0.154 5.79% -
Sample type
Plasma -
Assay type
Quantitative -
Sensitivity
0.00998 ng/ml -
Range
0.01 ng/ml - 10 ng/ml -
Recovery
97.75 %
Sample specific recovery Sample type Average % Range Spike = 97.7 89.3% - 106% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse
Does not react with: Sheep -
Product overview
Abcam’s uPA Total (URK) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of total urokinase plasminogen activator antigen in mouse plasma.
Mouse uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-murine uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then bound by the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-mouse uPA primary antibody 1 vial Anti-rabbit horseradish peroxidase secondary antibody 1 vial Mouse uPA standard 1 vial TMB Substrate Solution 1 x 10ml uPA Antibody pre-coated 96 well microplate (12 x 8 well strips) 1 x 96 tests -
Research areas
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Relevance
Urokinase type plasminogen activator (uPA) is secreted from cells as a precursor which is activated to the two chain form consisting of an A and B chain. The active high MW form is further processed by removal of an amino terminal fragment to an active low MW form (35 kDa). uPA is a serine protease that activates plasminogen toplasmin. High levels of uPA and plasminogen activator inhibitor type 1 (PAI 1) in breast cancer tissue extracts have been associated with rapid disease progression. The malignant phenotype of prostatic tumor cells correlates with the expression of both uPA and its cell membrane receptor (uPAR). -
Alternative names
- ATF
- BDPLT5
- EC 3.4.21.73
see all -
Database links
- Entrez Gene: 18792 Mouse
- SwissProt: P06869 Mouse
- Unigene: 4183 Mouse
Images
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Standard Curve.
Full range standard curve using ab198512 uPA Total (URK) Mouse ELISA Kit.
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Standard Curve.Linear range standard curve using ab198512 uPA Total (URK) Mouse ELISA Kit.