Human Factor XII ELISA Kit (ab192144)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.1 ng/ml
- Range: 0.23 ng/ml - 15 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Factor XII ELISA Kit
See all Factor XII kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% plasma 8 3% Inter-assay Sample n Mean SD CV% plasma 3 5.5% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.1 ng/ml -
Range
0.23 ng/ml - 15 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 93 88% - 98% Urine 107 104% - 110% Serum 109 98% - 118% Hep Plasma 105 100% - 109% EDTA Plasma 120 117% - 123% Cit plasma 110 108% - 116% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
As of April 2020, Human Factor XII ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.
Human Factor XII ELISA Kit (ab192144) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Factor XII protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Factor XII with 0.1 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Factor XII is a 80 kDa plasma serine protease involved in the coagulation cascade. Factor XII is activated by proteolysis and the product, Factor XIIa, goes on to activate Factor XI and prekallikrein in in vitro models of blood cogulation. Its involvement in the beginning of the coagulation cascade is only seen in in vitro studies, and is thought to have no effect on coagulation initiation in vivo. While Factor XII deficiency in humans is asymptomatic, Factor XII plays a role in later stages of clot formation. Deficiency of Factor XII affects the in vitro measurements of partial thromboplastin time in human blood, and its absence decreases the susceptibility of mice to thrombosis.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Factor XII Capture Antibody 1 x 600µl 10X Human Factor XII Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Human Factor XII Lyophilized Purified Protein (ab62423) 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. -
Involvement in disease
Defects in F12 are the cause of factor XII deficiency (FA12D) [MIM:234000]; also known as Hageman factor deficiency. This trait is an asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. F12 deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection).
Defects in F12 are the cause of hereditary angioedema type 3 (HAE3) [MIM:610618]; also known as estrogen-related HAE or hereditary angioneurotic edema with normal C1 inhibitor concentration and function. HAE is characterized by episodic local subcutaneous edema, and submucosal edema involving the upper respiratory and gastrointestinal tracts. HAE3 occurs exclusively in women and is precipitated or worsened by high estrogen levels (e.g., during pregnancy or treatment with oral contraceptives). It differs from HAE types 1 and 2 in that both concentration and function of C1 inhibitor are normal. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 fibronectin type-I domain.
Contains 1 fibronectin type-II domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsFactor XII is activated by kallikrein in alpha-factor XIIa, which is then further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of the NH2-terminal heavy chain (Coagulation factor XIIa heavy chain) and the COOH-terminal light chain (Coagulation factor XIIa light chain), connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, a light chain (Beta-factor XIIa part 2), corresponding to the COOH-terminal light chain (Coagulation factor XIIa light chain) and a nonapeptide (Beta-factor XIIa part 1).
O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Factor XII
- Beta factor XIIa part 1
- Beta factor XIIa part 2
see all -
Database links
- Entrez Gene: 2161 Human
- Omim: 610619 Human
- SwissProt: P00748 Human
- Unigene: 1321 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Factor XII standard curve in Sample Diluent NS.The Factor XII standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Factor XII in human serum, plasma and cell culture supernatant samples.The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:6,600, plasma (EDTA) 1:1,600, plasma (citrate) 1:1,600 and plasma (heparin) 1:8,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 25.0 µg/mL in serum, 21.7 µg/mL in plasma (EDTA), 21.0 µg/mL in plasma (citrate), and 32.1 µg/mL in plasma (heparin).
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Interpolated concentrations of native Factor XII in human urine samples.The concentrations of Factor XII were measured in duplicates, interpolated from the Factor XII standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 60.7 ng/mL in urine 12.5%.
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Serum from eight individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Factor XII concentration was determined to be 30.4 µg/mL with a range of 21.8 – 40.3 µg/mL.
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Sandwich ELISA - Human Factor XII ELISA Kit (ab192144)To learn more about the advantages of recombinant antibodies see here.
