Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23238-107] to GC1q R - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free
    See all GC1q R primary antibodies

  • Description

    Rabbit monoclonal [EPR23238-107] to GC1q R - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-P
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, A549, MCF7, WI-38 cell lysate; Rat, human and mouse spleen lysate; Human lung cancer lysate. IHC-P: Human, mouse and rat cerebral cortex. ICC/IF: HeLa and C2C12 cells. Flow: HeLa and C2C12 cells.

  • General notes

    ab270038 is the carrier-free version of ab270032. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling GC1q R with ab270032 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing mitochondrial staining in HeLa cell line. ab33985 Anti-COX IV antibody (human) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue). 

    Negative controls:
    -ve control 1: ab270032 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab150077 at 1/1000 dilution followed by ab33985 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270032).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling GC1q R with ab270032 at 1/2000 (0.248 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebral cortex (PMID: 23924515). The section was incubated with ab270032 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270032).

  • Flow Cytometry - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Flow Cytometry - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GC1q R with ab270032 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270032).

  • Flow Cytometry - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Flow Cytometry - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labeling GC1q R with ab270032 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270032).

  • Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Immunocytochemistry/ Immunofluorescence - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells labeling GC1q R with ab270032 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing mitochondrial staining in C2C12 cell line. ab33985 Anti-COX IV antibody (human) was used to counterstain tubulin at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue). 

    Negative controls:
    -ve control 1: ab270032 at 1/100 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab150077 at 1/1000 dilution followed by ab33985 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling GC1q R with ab270032 at 1/2000 (0.248 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebral cortex (PMID: 9414106). The section was incubated with ab270032 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling GC1q R with ab270032 at 1/2000 (0.248 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebral cortex (PMID: 9414106). The section was incubated with ab270032 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270032).

  • Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)
    Anti-GC1q R antibody [EPR23238-107] - BSA and Azide free (ab270038)

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