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Anti-uPA Receptor/U-PAR antibody [EPR22814-273] - BSA and Azide free (ab256763)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22814-273] to uPA Receptor/U-PAR - BSA and Azide free
  • Suitable for: Flow Cyt, IP, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-uPA Receptor/U-PAR antibody [EPR22814-273] - BSA and Azide free
    See all uPA Receptor/U-PAR primary antibodies

  • Description

    Rabbit monoclonal [EPR22814-273] to uPA Receptor/U-PAR - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, WBmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Mouse primary peripheral blood mononuclear cells. IP: LLC1 whole cell lysate.

  • General notes

    ab256763 is the carrier-free version of ab255815 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab256763 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] - BSA and Azide free (ab256763)
    Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] - BSA and Azide free (ab256763)

    uPA Receptor/U-PAR was immunoprecipitated from 0.35 mg LLC1 (mouse lung carcinoma) whole cell lysate 10μg with ab255815 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255815 at a 1/1000 dilution (0.59 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as at a 1/5000 dilution.

    Lane 1: LLC1 (mouse lung carcinoma) whole cell lysate 10μg.

    Lane 2: ab255815 IP in LLC1 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255815 in LLC1 whole cell lysate.

    Blocking/Dilution buffer : 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255815).

  • Flow Cytometry - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] - BSA and Azide free (ab256763)
    Flow Cytometry - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] - BSA and Azide free (ab256763)

    Flow Cytometric analysis of mouse PBMC (Mouse primary peripheral blood mononuclear cell) treated with 100ng/ml LPS for 16h, labeling uPA Receptor/U-PAR using ab255815 at a 1/600 dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary at a 1/2000 dilution.

    Cells were stained with rabbit IgG (Left) or ab255815 (Right). Then stained with anti-CD11b conjugated to Pacific blue.

    Gated on viable cells.

    Expression of uPAR on monocytes and granulocytes are observed in LPS-treated PBMC (PMID: 11447203).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255815).

  • Anti-uPA Receptor/U-PAR antibody [EPR22814-273] - BSA and Azide free (ab256763)
    Anti-uPA Receptor/U-PAR antibody [EPR22814-273] - BSA and Azide free (ab256763)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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