Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (ab255815)
![Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (ab255815)](https://www.abcam.com/ps/products/255/ab255815/Images/ab255815-3-anti-upa-receptor--upar-antibody-epr22814-273-western-blot-mouse.jpg "Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (ab255815)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22814-273] to uPA Receptor/U-PAR
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Mouse
Overview
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Product name
Anti-uPA Receptor/U-PAR antibody [EPR22814-273]
See all uPA Receptor/U-PAR primary antibodies -
Description
Rabbit monoclonal [EPR22814-273] to uPA Receptor/U-PAR -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseIP MouseWB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lung tissue lysate. LLC1 whole cell lysate. Flow Cyt: Mouse primary peripheral blood mononuclear cells. IP: LLC1 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22814-273 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (ab255815)All lanes : Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (ab255815) at 1/1000 dilution
Lane 1 : Mouse lung tissue lysate
Lane 2 : LLC1 (mouse lung carcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDauPAR is a glycoprotein. Full-length GPI-anchored uPAR (60kDa) and truncated soluble uPAR (45kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 19667118, PMID 24999729, PMID: 11535128).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 81 secs; Lane 2: 158 secs.
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![Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (ab255815)](https://www.abcam.com/ps/products/255/ab255815/Images/ab255815-2-anti-upa-receptor--upar-antibody-epr22814-273-llc1-mouse.jpg)
Immunoprecipitation - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (ab255815)uPA Receptor/U-PAR was immunoprecipitated from 0.35 mg LLC1 (mouse lung carcinoma) whole cell lysate 10μg with ab255815 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255815 at a 1/1000 dilution (0.59 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at a 1/5000 dilution.
Lane 1: LLC1 (mouse lung carcinoma) whole cell lysate 10μg.
Lane 2: ab255815 IP in LLC1 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255815 in LLC1 whole cell lysate.
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time: 3 seconds.
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![Flow Cytometry - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (ab255815)](https://www.abcam.com/ps/products/255/ab255815/Images/ab255815-1-anti-upa-receptor--upar-antibody-epr22814-273-flowcytometry-pbmc-mouse.jpg)
Flow Cytometry - Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (ab255815)Flow Cytometric analysis of mouse PBMC (Mouse primary peripheral blood mononuclear cell) treated with 100ng/ml LPS for 16h, labeling uPA Receptor/U-PAR using ab255815 at a 1/600 dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary at a 1/2000 dilution.
Cells were stained with rabbit IgG (Left) or ab255815 (Right). Then stained with anti-CD11b conjugated to Pacific blue.
Gated on viable cells.
Expression of uPAR on monocytes and granulocytes are observed in LPS-treated PBMC (PMID: 11447203).
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![Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (ab255815)](https://www.abcam.com/ps/products/255/ab255815/Images/ab255815-4-benefits-of-recombinant-antibodies.png)
Anti-uPA Receptor/U-PAR antibody [EPR22814-273] (ab255815)
