Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (ab240971)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15870] to NDUFAF5 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free
    See all NDUFAF5 primary antibodies

  • Description

    Rabbit monoclonal [EPR15870] to NDUFAF5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human endometrium and breast carcinoma tissues. ICC/IF: NIH/3T3 cells. Flow Cyt: NIH/3T3 cells. IP: 293 cell lysate.

  • General notes

    Ab240971 is the carrier-free version of ab192235. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240971 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15870

  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (ab240971)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (ab240971)

    Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling NDUFAF5 with ab192235 at 1/1000 dilution, followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192235).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (ab240971)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (ab240971)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling NDUFAF5 with ab192235 at 1/1000 dilution, followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192235).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (ab240971)
    Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (ab240971)

    Immunofluorescent analysis of NIH 3T3 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling NDUFAF5 with ab192235 at 1/400 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
    Negative controls: anti-NDUFAF5 at 1/400 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192235).

  • Immunoprecipitation - Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (ab240971)
    Immunoprecipitation - Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (ab240971)

    Western blot analysis of pellet from 293 cell lysate immunoprecipitated using ab192235 at 1/100 dilution (lane 1) or PBS control (lane 2).
    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192235).

  • Flow Cytometry - Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (ab240971)
    Flow Cytometry - Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (ab240971)

    Flow cytometry analysis of NIH/3T3 (mouse embryo) cells labelling NDUFAF5 (red) with purified ab192235 at dilution of 1/200. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit Monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192235).

  • Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (ab240971)
    Anti-NDUFAF5 antibody [EPR15870] - BSA and Azide free (ab240971)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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