Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15870] to NDUFAF5 - C-terminal
  • Suitable for: IHC-P, ICC/IF, IP, WB, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-NDUFAF5 antibody [EPR15870] - C-terminal
    See all NDUFAF5 primary antibodies

  • Description

    Rabbit monoclonal [EPR15870] to NDUFAF5 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, 293, HeLa, Jurkat, Human skeletal muscle and fetal liver, mouse spleen, brain, heart, kidney, NIH 3T3 and Raw264.7 cell lysates; Human endometrium and Human breast carcinoma tissues; NIH 3T3 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15870

  • Isotype

    IgG

Images

  • Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
    Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
    All lanes : Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235) at 1/5000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 38 kDa
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)

    Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling NDUFAF5 with ab192235 at 1/1000 dilution, followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
    Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)

    Immunofluorescent analysis of NIH 3T3 cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling NDUFAF5 with ab192235 at 1/400 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
    Negative controls: anti-NDUFAF5 at 1/400 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution.

  • Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
    Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
    All lanes : Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 38 kDa
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
    Western blot - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
    All lanes : Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : RAW 264.7 lysate
    Lane 6 : NIH 3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 38 kDa
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling NDUFAF5 with ab192235 at 1/1000 dilution, followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
    Flow Cytometry - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)

    Flow cytometry analysis of NIH/3T3 (mouse embryo) cells labelling NDUFAF5 (red) with purified ab192235 at dilution of 1/200. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit Monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • Immunoprecipitation - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
    Immunoprecipitation - Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)

    Western blot analysis of pellet from 293 cell lysate immunoprecipitated using ab192235 at 1/100 dilution (lane 1) or PBS control (lane 2).
    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution.

  • Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)
    Anti-NDUFAF5 antibody [EPR15870] - C-terminal (ab192235)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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