Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4392(2)] to LAG-3 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free
    See all LAG-3 primary antibodies

  • Description

    Rabbit monoclonal [EPR4392(2)] to LAG-3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human Hodgkins lymphoma and Human tonsil tissues

  • General notes

    ab209740 is the carrier-free version of ab180187 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab209740 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.This product was previously labelled as Lymphocyte Activation Gene 3.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue sections labeling LAG-3 with ab180187 at 1/8000 dilution (0.13 µg/mL). Tissue was pre-treated with Tris EDTA buffer, pH 9. Detection was carried out using ab209101, Rabbit specific IHC polymer detection kit HRP/DAB. Micrographs were captured using the Leica AperioAT2 using the 20x objective.

    LAG-3 does not stain in white matter. Glial cells, myelinated axons, and capillaries are negative for LAG-3.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180187).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)

    Clone EPR4392(2) (ab209740) has been successfully conjugated by Abcam. This image was generated using Anti-LAG-3 antibody [EPR4392(2)] (Alexa Fluor® 647). Please refer to ab225278 for protocol details.

    IHC image of LAG-3 staining in a section of formalin-fixed paraffin-embedded human Hodgkin's lymphoma*.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110oC for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab225278 at 1/100 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)

    Clone EPR4392(2) (ab209740) has been successfully conjugated by Abcam. This image was generated using Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (Alexa Fluor® 488). Please refer to ab225277 for protocol details.

    IHC image of LAG-3 - C-terminal staining in a section of formalin-fixed paraffin-embedded human Hodgkin's lymphoma*.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110oC for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab225277 at 1/250 dilution (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue sections labeling LAG-3 with ab180187 at 1/8000 dilution (0.13 µg/mL). Tissue was pre-treated with Tris EDTA buffer, pH 9. Detection was carried out using ab209101, Rabbit specific IHC polymer detection kit HRP/DAB. Micrographs were captured using the Leica AperioAT2 using the 20x objective.

    Strong LAG-3 staining in neuron cell bodies and weak staining in neuropil.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180187).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)

    Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling LAG-3 with ab180187 at 1/1000.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180187).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)

    This IHC data was generated using the same anti-LAG3 antibody clone, EPR4392(2), in a different buffer formulation (cat# ab180187).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue sections labeling LAG-3 with ab180187 at 1/8000 dilution (0.13 µg/mL). Tissue was pre-treated with Tris EDTA buffer, pH 9. Detection was carried out using ab209101, Rabbit specific IHC polymer detection kit HRP/DAB. Micrographs were captured using the Leica AperioAT2 using the 20x objective.

    Lymphocyte Activation Gene 3 staining in gray matter. Neuron cell bodies are strongly labeled and moderate staining in neuropil.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)

    This IHC data was generated using the same anti-LAG3 antibody clone, EPR4392(2), in a different buffer formulation (cat# ab180187).

    Immunohistochemical analysis of paraffin embedded Human Hodgkins lymphoma tissue labeling LAG-3 with ab180187 at 1/1000.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)
    Anti-LAG-3 antibody [EPR4392(2)] - BSA and Azide free (ab209740)

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