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Immunology Adaptive Immunity T Cells CD

Anti-LAG-3 antibody [EPR20294-96] (ab228411)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-LAG-3 antibody [EPR20294-96] (ab228411)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20294-96] to LAG-3
  • Suitable for: Flow Cyt
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-LAG-3 antibody [EPR20294-96]
    See all LAG-3 primary antibodies
  • Description

    Rabbit monoclonal [EPR20294-96] to LAG-3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Mouse primary splenocytes treated with 2.5g/ml concanavalin A for 72h.
  • General notes

    This product was previously labelled as Lymphocyte Activation Gene 3.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20294-96
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Cell Type Markers
    • CD
    • NK Cells
    • Immunology
    • Secreted Molecules
    • Other secreted molecules
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Images

  • Flow Cytometry - Anti-LAG-3 antibody [EPR20294-96] (ab228411)
    Flow Cytometry - Anti-LAG-3 antibody [EPR20294-96] (ab228411)

    Flow cytometric analysis of mouse primary splenocytes treated with 2.5μg/ml concanavalin A for 72h labeling LAG-3 with ab228411 at 1/600 dilution (right) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)(left). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Mouse primary splenocytes treated with 2.5μg/ml concanavalin A for 72h, then surface stained with anti-mouse CD3 (Y axis) and ab228411. Only CD3+ population gives a positive signal for Lymphocyte Activation Gene 3. Gated on total viable cells.

  • Anti-LAG-3 antibody [EPR20294-96] (ab228411)
    Anti-LAG-3 antibody [EPR20294-96] (ab228411)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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