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Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4392(2)] to LAG-3 - C-terminal
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-LAG-3 antibody [EPR4392(2)] - C-terminal
    See all LAG-3 primary antibodies

  • Description

    Rabbit monoclonal [EPR4392(2)] to LAG-3 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human LAG-3 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P18627

  • Positive control

    • Human Hodgkins lymphoma and Human tonsil tissues

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product was previously labelled as Lymphocyte Activation Gene 3.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)

    Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling LAG-3 with ab180187 at 1/1000.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)

    Immunohistochemical analysis of paraffin embedded Human Hodgkins lymphoma tissue labeling LAG-3 with ab180187 at 1/1000.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue sections labeling LAG-3 with ab180187 at 1/8000 dilution (0.13 µg/mL). Tissue was pre-treated with Tris EDTA buffer, pH 9. Detection was carried out using ab209101, Rabbit specific IHC polymer detection kit HRP/DAB. Micrographs were captured using the Leica AperioAT2 using the 20x objective.

    Lymphocyte Activation Gene 3 staining in gray matter. Neuron cell bodies are strongly labeled and moderate staining in neuropil.

     

     

     

    　

     

     

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue sections labeling LAG-3 with ab180187 at 1/8000 dilution (0.13 µg/mL). Tissue was pre-treated with Tris EDTA buffer, pH 9. Detection was carried out using ab209101, Rabbit specific IHC polymer detection kit HRP/DAB. Micrographs were captured using the Leica AperioAT2 using the 20x objective.

    Strong Lymphocyte Activation Gene 3 staining in neuron cell bodies and weak staining in neuropil.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue sections labeling LAG-3 with ab180187 at 1/8000 dilution (0.13 µg/mL). Tissue was pre-treated with Tris EDTA buffer, pH 9. Detection was carried out using ab209101, Rabbit specific IHC polymer detection kit HRP/DAB. Micrographs were captured using the Leica AperioAT2 using the 20x objective.

    LAG-3 does not stain in white matter. Glial cells, myelinated axons, and capillaries are negative for LAG-3.

     

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)
    Anti-LAG-3 antibody [EPR4392(2)] - C-terminal (ab180187)

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