Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Entactin/NID antibody [EPR22414-125] (ab254325)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22414-125] to Entactin/NID
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Entactin/NID antibody [EPR22414-125]
    See all Entactin/NID primary antibodies

  • Description

    Rabbit monoclonal [EPR22414-125] to Entactin/NID

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant human Entactin/NID protein (aa29 -1114); Human kidney, skeletal muscle, lung and placenta tissue lysate; Mouse heart, lung, skeletal muscle, kidney tissue lysate. IHC-P: Mouse cardiac tissue and rat cardiac muscle tissue. IHC-Fr: Mouse and rat heart tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Entactin/NID with ab254325 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on basement membrane of rat cardiac muscle (PMID: 10958695). The section was incubated with ab254325 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Entactin/NID with ab254325 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on basement membrane of mouse cardiac muscle (PMID: 10958695). The section was incubated with ab254325 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
    Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen mouse heart tissue labeling Entactin/NID with ab254325 at 1/500 dilution (green), followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on the basement membrane of cardiac muscle (PMID: 12215539) is observed. The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
    Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)

    Immunohistochemical analysis of 4% Paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Entactin/NID with ab254325 at 1/500 dilution (green), followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on the basement membrane of cardiac muscle (PMID: 12215539) is observed. The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

  • Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
    Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
    All lanes : Anti-Entactin/NID antibody [EPR22414-125] (ab254325) at 1/1000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse lung tissue lysate
    Lane 3 : Mouse skeletal muscle tissue lysate
    Lane 4 : Mouse kidney tissue lysate
    Lane 5 : Rat lung tissue lysate
    Lane 6 : Rat skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 136 kDa


    Exposure time: 3 seconds


    Blocking/diluting buffer and concentration: 5% NFDM/TBST 

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:3084254,12215539, 22952693).

  • Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
    Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
    All lanes : Anti-Entactin/NID antibody [EPR22414-125] (ab254325) at 1/1000 dilution

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Human skeletal muscle tissue lysate
    Lane 3 : Human placenta tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 136 kDa
    Observed band size: 160, 100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:3084254, 12215539, 22952693).

  • Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
    Western blot - Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
    Anti-Entactin/NID antibody [EPR22414-125] (ab254325) at 1/1000 dilution + Recombinant human Entactin/NID protein (aa29 -1114) 20 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 136 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

  • Anti-Entactin/NID antibody [EPR22414-125] (ab254325)
    Anti-Entactin/NID antibody [EPR22414-125] (ab254325)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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