Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22414-125] to Entactin/NID - BSA and Azide free
  • Suitable for: IHC-Fr, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free
    See all Entactin/NID primary antibodies

  • Description

    Rabbit monoclonal [EPR22414-125] to Entactin/NID - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse cardiac tissue and rat cardiac muscle tissue. IHC-Fr: Mouse and rat heart tissue.

  • General notes

    Ab254994 is the carrier-free version of ab254325. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab254994 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)
    Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen rat heart tissue labeling Entactin/NID with ab254325 at 1/500 dilution (green), followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on the basement membrane of cardiac muscle (PMID: 12215539) is observed. The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254325).

  • Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)
    Immunohistochemistry (Frozen sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)

    Immunohistochemical analysis of 4% paraformaldehyde fixed, 0.2% Triton X-100 permeabilized frozen mouse heart tissue labeling Entactin/NID with ab254325 at 1/500 dilution (green), followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining on the basement membrane of cardiac muscle (PMID: 12215539) is observed. The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254325).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Entactin/NID with ab254325 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on basement membrane of rat cardiac muscle (PMID: 10958695). The section was incubated with ab254325 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254325).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Entactin/NID with ab254325 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on basement membrane of mouse cardiac muscle (PMID: 10958695). The section was incubated with ab254325 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254325).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)
    Anti-Entactin/NID antibody [EPR22414-125] - BSA and Azide free (ab254994)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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