Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR027F] to LAG-3 - BSA free
  • Suitable for: WB, ICC, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-LAG-3 antibody [BLR027F] - BSA free
    See all LAG-3 primary antibodies

  • Description

    Rabbit monoclonal [BLR027F] to LAG-3 - BSA free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human LAG-3 aa 500-525 (C terminal). The exact sequence is proprietary. NP_002277.4 and Gene ID 3902.
    Database link: P18627

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

    This product was previously labelled as Lymphocyte Activation Gene 3.

    ab272078 is the carrier-free version of ab243884. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Properties

Images

  • Immunoprecipitation - Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)
    Immunoprecipitation - Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)

    LAG-3 was immunoprecipitated from 0.5 mg HDLM-2 whole cell lysate with ab243884 at 12 µl per reaction. Western blot was performed on the immunoprecipitate using ab243884 at 1/1000 dilution.

    Lane 1: ab243884 IP in HDLM-2 whole cell lysate.

    Lane 2: Control IgG IP in HDLM-2 whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243884).

  • Immunocytochemistry - Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)
    Immunocytochemistry - Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)

    Formalin-fixed, paraffin-embedded HDLM-2 cells labeling LAG-3 using ab243884 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243884).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 using ab243884 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

    This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243884).

  • Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)
    Anti-LAG-3 antibody [BLR027F] - BSA free (ab272078)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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