Anti-TIAL1 antibody [EPR11323(B)] (ab169547)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11323(B)] to TIAL1
- Suitable for: WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-TIAL1 antibody [EPR11323(B)]
See all TIAL1 primary antibodies -
Description
Rabbit monoclonal [EPR11323(B)] to TIAL1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human TIAL1.
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Positive control
- WB: HeLa, Jurkat and K562 cell lysates. IHC-P: Human breast and ovarian carcinoma tissues. Flow Cyt: K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11323(B) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-TIAL1 antibody [EPR11323(B)] (ab169547) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TIAL1 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K-562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab169547 observed at 49 kDa. Red - loading control ab8245 observed at 36 kDa.
ab169547 Anti-TIAL1 antibody [EPR11323(B)] was shown to specifically react with TIAL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265793 (knockout cell lysate ab257743) was used. Wild-type and TIAL1 knockout samples were subjected to SDS-PAGE. ab169547 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling TIAL1 with ab169547 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAL1 antibody [EPR11323(B)] (ab169547)
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling TIAL1 with ab169547 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow cytometric analysis of permeabilized K562 cells labeling TIAL1 with ab169547 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
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All lanes : Anti-TIAL1 antibody [EPR11323(B)] (ab169547) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDa
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