Anti-GARNL1 antibody [EPR13592-38] (ab182570)
![Anti-GARNL1 antibody [EPR13592-38] (ab182570)](https://www.abcam.com/ps/products/182/ab182570/Images/ab182570-213858-anti-garnl1-antibody-epr13592-38-immunofluorescence.jpg "Anti-GARNL1 antibody [EPR13592-38] (ab182570)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13592-38] to GARNL1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-GARNL1 antibody [EPR13592-38]
See all GARNL1 primary antibodies -
Description
Rabbit monoclonal [EPR13592-38] to GARNL1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293 and HeLa cell lysate; Human kidney tissue; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13592-38 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GARNL1 antibody [EPR13592-38] (ab182570)
Immunofluorescence analysis of HeLa cells labeling GARNL1 using ab182570 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with -20℃ Acetone. Counterstain: DAPI.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARNL1 antibody [EPR13592-38] (ab182570)](https://www.abcam.com/ps/products/182/ab182570/Images/ab182570-213854-anti-garnl1-antibody-epr13592-38-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARNL1 antibody [EPR13592-38] (ab182570)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GARNL1 using ab182570 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG was used as a secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-GARNL1 antibody [EPR13592-38] (ab182570)](https://www.abcam.com/ps/products/182/ab182570/Images/ab182570-213852-anti-garnl1-antibody-epr13592-38-western-blot.jpg)
Western blot - Anti-GARNL1 antibody [EPR13592-38] (ab182570)Anti-GARNL1 antibody [EPR13592-38] (ab182570) at 1/100000 dilution + HeLa cell lysate at 1/10 dilution
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 230 kDa
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![Western blot - Anti-GARNL1 antibody [EPR13592-38] (ab182570)](https://www.abcam.com/ps/products/182/ab182570/Images/ab182570-213851-anti-garnl1-antibody-epr13592-38-western-blot.jpg)
Western blot - Anti-GARNL1 antibody [EPR13592-38] (ab182570)Anti-GARNL1 antibody [EPR13592-38] (ab182570) at 1/100000 dilution + 293 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 230 kDa
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![Anti-GARNL1 antibody [EPR13592-38] (ab182570)](https://www.abcam.com/ps/products/182/ab182570/Images/ab182570-5-benefits-of-recombinant-antibodies.png)
Anti-GARNL1 antibody [EPR13592-38] (ab182570)
