Anti-ZAP70 antibody [ZAP-03] (ab38871)
![Anti-ZAP70 antibody [ZAP-03] (ab38871)](https://www.abcam.com/ps/products/38/ab38871/Images/ab38871-51835-anti-zap70-antibody-zap-03-flow-cytometry.jpg "Anti-ZAP70 antibody [ZAP-03] (ab38871)")
Key features and details
- Mouse monoclonal [ZAP-03] to ZAP70
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ZAP70 antibody [ZAP-03]
See all ZAP70 primary antibodies -
Description
Mouse monoclonal [ZAP-03] to ZAP70 -
Host species
Mouse -
Specificity
ab38871 recognises ZAP-70, a 70 kDa protein tyrosine kinase expressed in T and NK cells. -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment representing C-terminal part (160 amino acids) of human ZAP-70.
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Positive control
- HPB-ALL human peripheral blood leukemia T-cell line
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
ZAP-03 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-ZAP70 antibody [ZAP-03] (ab38871)Flow Cytometry analysis of the human peripheral blood of a patient with B-cell chronic lymphatic leukemia (B-CLL) using antibody ab38871; Dot Plot diagram shows the distribution of lymphocyte populations: T-lymphocytes (green), B-lymphocytes (blue) and NK cells (red).
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![Western blot - Anti-ZAP70 antibody [ZAP-03] (ab38871)](https://www.abcam.com/ps/products/38/ab38871/Images/ab38871-51834-anti-zap70-antibody-zap-03-western-blot.jpg)
Western blot - Anti-ZAP70 antibody [ZAP-03] (ab38871)Lane 1 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 4 µg/ml
Lane 2 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 2 µg/ml
Lane 3 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 1 µg/ml
Lane 4 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 0.5 µg/ml
Lane 5 : ZAP-70 specific antibody 1E7.2 at 1 µg/ml
Lane 6 : Isotypic control PPV-06 (mouse IgG1).
All lanes : HPB-ALL peripheral blood leukemia T-cell line
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Additional bands at: 55 kDa (possible IgG), 55 kDa (possible degradation product)
Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line; SDS-PAGE; 10% separating gel, 4% stacking gel; Reducing conditions. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products. Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line; SDS-PAGE; 10% separating gel, 4% stacking gel. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products. -
![Western blot - Anti-ZAP70 antibody [ZAP-03] (ab38871)](https://www.abcam.com/ps/products/38/ab38871/Images/ab38871-51833-anti-zap70-antibody-zap-03-western-blot.jpg)
Western blot - Anti-ZAP70 antibody [ZAP-03] (ab38871)Lanes 1-2 : Anti-ZAP70 antibody [ZAP-03] (ab38871) at 0.5 µg/ml
Lanes 3-4 : Isotypic control PPV-06 (mouse IgG1)
Lanes 1 & 3 : HPB-ALL cell line
Lanes 2 & 4 : RAMOS cell line
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Western Blotting analysis of HPB-ALL peripheral blood leukemia T-cell line and RAMOS human Burkitt lymphoma cell line; SDS-PAGE; 10% separating gel, 4% stacking gel.
