Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Immunology Adaptive Immunity T Cells CD

Anti-ZAP70 antibody [E267] (ab32410)

Price and availability

298 185 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-ZAP70 antibody [E267] (ab32410)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E267] to ZAP70
  • Suitable for: WB
  • Reacts with: Human

You may also be interested in

Product image
Alexa Fluor® 647 Anti-CD272/BTLA antibody [EPR20539] (ab237328)
Product image
Recombinant mouse CD134 / OX40L receptor protein (Active) (ab221028)
Product image
Anti-CD10 antibody - BSA and Azide free (Detector) (ab245021)
Product image
Alexa Fluor® 594 Anti-CD90 / Thy1 antibody [EPR3133] (ab202512)

Overview

  • Product name

    Anti-ZAP70 antibody [E267]
    See all ZAP70 primary antibodies
  • Description

    Rabbit monoclonal [E267] to ZAP70
  • Host species

    Rabbit
  • Specificity

    ab32410 recognises Zap-70, a Syk-family protein tyrosine kinase.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human ZAP70 aa 300-400 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat whole cell lysate (ab7899)
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    E267
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other

Images

  • Western blot - Anti-ZAP70 antibody [E267] (ab32410)
    Western blot - Anti-ZAP70 antibody [E267] (ab32410)
    All lanes : Anti-ZAP70 antibody [E267] (ab32410) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
    Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed)

    Predicted band size: 70 kDa



    Anti-GAPDH antibody, ab8245 (1/20000) was used as a primary antibody for the loading control and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed, ab216776 (1/10000) was used as a loading control secondary antibody.

    Lanes 1-2: Merged signal (red and green). Green – ab32410 observed at 70 kDa. Red - loading control ab8245 observed at 36 kDa.
     ab32410 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    The expression profile observed in Raji is consistent with the literature (PMID: 25275600).

    Negative control: Raji (PMID: 25275600)

  • Western blot - Anti-ZAP70 antibody [E267] (ab32410)
    Western blot - Anti-ZAP70 antibody [E267] (ab32410)
    Anti-ZAP70 antibody [E267] (ab32410) at 1/500 dilution + Jurkat cell lysates

    Predicted band size: 70 kDa
    Observed band size: 70 kDa



    Western blot analysis on Jurkat Cell lysate.
  • Anti-ZAP70 antibody [E267] (ab32410)
    Anti-ZAP70 antibody [E267] (ab32410)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-ZAP70 antibody [E267] (ab32410)

  •  
  • Product image

    Anti-ZAP70 antibody [YE291] (ab32429)

    Applications: Flow Cyt, IHC-P, IP, WB

  •  
  • Product image

    Anti-ZAP70 antibody (ab227237)

    Applications: ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Anti-ZAP70 (phospho Y319) antibody (ab264260)

    Applications: Flow Cyt, IP

  •  
  • Product image

    Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)

    Applications: Dot, Flow Cyt, ICC/IF, WB

  •  
  • Product image

    Anti-ZAP70 (phospho Y493) antibody (ab194800)

    Applications: IHC-P, WB

  •  
  • Product image

    Anti-ZAP70 antibody (ab245441)

    Applications: IP, WB

  •  
  • Product image

    Anti-ZAP70 antibody - C-terminal (ab231452)

    Applications: IHC-P, WB

  •  
  • Product image

    Alexa Fluor® 647 Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab199497)

    Applications: ICC/IF

  •  
  • Product image

    HRP Anti-ZAP70 antibody [YE291] (ab208713)

    Applications: WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-ERp57 antibody [EPR10679(B)] (ab154197)

  •  
  • Product image

    Anti-Chk2 antibody [EPR5528] (ab133505)

  •  
  • Product image

    Anti-Alanine Transaminase antibody [EPR19616] (ab202083)

  •  
  • Product image

    Anti-alpha 1 Spectrin antibody [EPR9300] (ab139403)

  •  
  • Product image

    Anti-MRC2/ENDO180 antibody (ab70132)

  •  
  • Product image

    Anti-CPSF2/CPSF100 antibody (ab81553)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.