HRP Anti-ZAP70 antibody [YE291] (ab208713)
![HRP Anti-ZAP70 antibody [YE291] (ab208713)](https://www.abcam.com/ps/products/208/ab208713/Images/ab208713-270858-anti-zap70-antibody-ye291-hrp-western-blot.jpg "HRP Anti-ZAP70 antibody [YE291] (ab208713)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [YE291] to ZAP70
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
-
Product name
HRP Anti-ZAP70 antibody [YE291]
See all ZAP70 primary antibodies -
Description
HRP Rabbit monoclonal [YE291] to ZAP70 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide within Human ZAP70 (C terminal). The exact sequence is proprietary.
Database link: P43403 -
Positive control
- WB: Jurkat whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
YE291 -
Isotype
IgG -
Research areas
Images
-
Western blot - HRP Anti-ZAP70 antibody [YE291] (ab208713)HRP Anti-ZAP70 antibody [YE291] (ab208713) at 1/5000 dilution + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208713 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![HRP Anti-ZAP70 antibody [YE291] (ab208713)](https://www.abcam.com/ps/products/208/ab208713/Images/ab208713-2-benefits-of-recombinant-antibodies.png)
HRP Anti-ZAP70 antibody [YE291] (ab208713)
