HRP Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab199245)
![HRP Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab199245)](https://www.abcam.com/ps/products/199/ab199245/Images/ab199245-257915-anti-zap70-phospho-y292-antibody-epr1073-hrp-western-blot.jpg "HRP Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab199245)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR1073] to ZAP70 (phospho Y292)
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-ZAP70 (phospho Y292) antibody [EPR1073]
See all ZAP70 primary antibodies -
Description
HRP Rabbit monoclonal [EPR1073] to ZAP70 (phospho Y292) -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat whole cell lysate treated with pervanadate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1073 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab199245)All lanes : HRP Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab199245) at 1/2000 dilution
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate treated with Pervanadate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate treated with Pervanadate, the membrane was then incubated with Alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 15 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk before being incubated with ab199245 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab199245)](https://www.abcam.com/ps/products/199/ab199245/Images/ab199245-3-benefits-of-recombinant-antibodies.png)
HRP Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab199245)
