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Immunology Adaptive Immunity T Cells CD

Anti-ZAP70 antibody (ab245441)

Anti-ZAP70 antibody (ab245441)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to ZAP70
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ZAP70 antibody
    See all ZAP70 primary antibodies
  • Description

    Rabbit polyclonal to ZAP70
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human ZAP70 aa 564-614. The exact sequence is proprietary. NP_001070.2
    Database link: P43403

  • Positive control

    • WB: Jurkat whole cell lysate. IP: ZAP70 IP in Jurkat whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245441 was affinity purified using an epitope specific to ZAP70 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other

Images

  • Western blot - Anti-ZAP70 antibody (ab245441)
    Western blot - Anti-ZAP70 antibody (ab245441)
    All lanes : Anti-ZAP70 antibody (ab245441) at 0.1 µg/ml

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 5 µg

    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-ZAP70 antibody (ab245441)
    Immunoprecipitation - Anti-ZAP70 antibody (ab245441)

    ZAP70 was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
    ab245441 used for IP at 3 µg/mg lysate. For WB 1 µg/ml.

    Lane 1: ab245441 IP in Jurkat whole cell lysate.
    Lane 2: Control IgG IP in Jurkat whole cell lysate.
    Chemiluminescence detection: 10 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: WB

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    HRP Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab199245)

    Applications: WB

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    Applications: Dot, Flow Cyt, ICC/IF, WB

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