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Immunology Adaptive Immunity T Cells CD

Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)

Price and availability

298 185 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1073] to ZAP70 (phospho Y292)
  • Suitable for: WB, Flow Cyt, ICC/IF, Dot blot
  • Reacts with: Human

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Overview

  • Product name

    Anti-ZAP70 (phospho Y292) antibody [EPR1073]
    See all ZAP70 primary antibodies
  • Description

    Rabbit monoclonal [EPR1073] to ZAP70 (phospho Y292)
  • Host species

    Rabbit
  • Specificity

    ab76501 only detects ZAP70 when phosphorylated at Tyrosine 292.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ZAP70 (phospho Y292). The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899) treated with pervanadate. ICC/IF: Untreated and pervanadate treated Jurkat cells. Flow Cyt: Untreated and pervanadate treated Jurkat cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR1073
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other

Images

  • Western blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
    Western blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
    All lanes : Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501) at 1/5000 dilution

    Lane 1 : Untreated Jurkat (human acute T cell leukemia) whole cell lysates
    Lane 2 : Jurkat (human acute T cell leukemia) treated with pervanadate whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
    Dot Blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)

    Dot blot analysis of varying amounts of ZAP70 (pY292) phospho peptide (Lane 1) and ZAP70 non-phospho peptide (Lane 2) using ab76501 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

    Exposure time: 3 minutes.

  • Immunocytochemistry/ Immunofluorescence - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
    Immunocytochemistry/ Immunofluorescence - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)

    Immunocytochemistry/Immunofluorescence analysis of untreated and pervanadate treated Jurkat (human acute T cell leukemia) cells labelling ZAP70 (phospho Y292) with ab76501 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Treated samples were starved overnight followed by incubation with 1mM pervanadate for 30 minutes. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Negative control using secondary antibody only.

    Image shows increased cytoplasmic staining after pervanadate treatment.

  • Flow Cytometry - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
    Flow Cytometry - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
    Flow cytometric analysis of permeabilized Jurkat cells, untreated (green), pervanadate treated (red) stained with ab76501, at 1/20 dilution (blue) and pervanadate-treated Jurkat cells using the same antibody preincubated with phospho- ZAP70 (Tyr 292) peptide (blue) or non-phospho- ZAP70 (Tyr 292)).
  • Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)
    Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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