Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)
![Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)](https://www.abcam.com/ps/products/245/ab245687/Images/ab245687-336943-anti-il-2-receptor-alpha-antibody-basiliximab-immunohistochemistry.jpg "Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)")
Key features and details
- Rabbit monoclonal [Basiliximab] to IL-2 Receptor alpha
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Rat, Human, Cynomolgus monkey, Rhesus monkey
- Isotype: IgG
Overview
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Product name
Anti-IL-2 Receptor alpha antibody [Basiliximab]
See all IL-2 Receptor alpha primary antibodies -
Description
Rabbit monoclonal [Basiliximab] to IL-2 Receptor alpha -
Host species
Rabbit -
Specificity
This antibody recognizes human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit. Binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R (αβγc) – T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P Rat
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Immunogen
Tissue, cells or virus corresponding to IL-2 Receptor alpha. CTC 16 cells.
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Positive control
- IHC-P: Rat spleen and thymus tissue. Flow Cyt: Human, rhesus monkey and cynomolgus monkey lymphocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Basiliximab -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)
Formalin-fixed rat spleen tissue stained for IL-2 Receptor alpha with ab245687 at 5 µg/ml in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)](https://www.abcam.com/ps/products/245/ab245687/Images/ab245687-336944-anti-il-2-receptor-alpha-antibody-basiliximab-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)Formaldehyde-fixed rat thymus tissue stained for IL-2 Receptor alpha with ab245687 at 5 µg/ml in immunohistochemical analysis. Detected with an FITC-conjugated secondary antibody.
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![Flow Cytometry - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)](https://www.abcam.com/ps/products/245/ab245687/Images/ab245687-336945-anti-il-2-receptor-alpha-antibody-basiliximab-flow-cytometry.jpg)
Flow Cytometry - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)Human lymphocytes were stained with an isotype control (Panel A) or ab245687 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 conjugated donkey anti-rabbit antibody (ab150073).
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![Flow Cytometry - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)](https://www.abcam.com/ps/products/245/ab245687/Images/ab245687-336946-anti-il-2-receptor-alpha-antibody-basiliximab-flow-cytometry.jpg)
Flow Cytometry - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)Rhesus monkey lymphocytes were stained with an isotype control (Panel A) or ab245687 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 conjugated donkey anti-rabbit antibody (ab150073).
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![Flow Cytometry - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)](https://www.abcam.com/ps/products/245/ab245687/Images/ab245687-336947-anti-il-2-receptor-alpha-antibody-basiliximab-flow-cytometry.jpg)
Flow Cytometry - Anti-IL-2 Receptor alpha antibody [Basiliximab] (ab245687)Cynomolgus monkey lymphocytes were stained with an isotype control (Panel A) or ab245687 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 conjugated donkey anti-rabbit antibody (ab150073).
