Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP176] to IL-2 Receptor alpha
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-IL-2 Receptor alpha antibody [SP176]
    See all IL-2 Receptor alpha primary antibodies

  • Description

    Rabbit monoclonal [SP176] to IL-2 Receptor alpha

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MOLT-4, HDLM-2 and WEHI-231 whole cell lysate. IHC-P: Human endometrium and tonsil tissue. ICC/IF: HDLM-2 cells

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)

    Formalin-fixed, paraffin-embedded human endometrium tissue stained for IL2 Receptor alpha using ab231441 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 0.433 µg/ml + HDLM-2 (Human Hodgkin lymphoma) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 31 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer and concentration: 5% NFDM /TBST
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)

    Immunocytochemistry/ Immunofluorescence analysis of HDLM-2 (Human Hodgkin lymphoma) cells labeling IL-2 Receptor alpha with purified ab231441 at 1/50 (9.5µg/mL). Cells were fixed in 100% methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 0.433 µg/ml + WEHI-231 (Mouse B cell lymphoma B lymphocyte) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 31 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking/Diluting buffer and concentration: 5% NFDM /TBST
  • Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 1/200 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 31 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for IL2 Receptor alpha using ab231441 at 1/100 dilution in immunohistochemical analysis.

  • Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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