Anti-ZAP70 (phospho Y493) antibody (ab194800)
Key features and details
- Rabbit polyclonal to ZAP70 (phospho Y493)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ZAP70 (phospho Y493) antibody
See all ZAP70 primary antibodies -
Description
Rabbit polyclonal to ZAP70 (phospho Y493) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human ZAP70 (phospho Y493). The exact sequence is proprietary.
Database link: P43403
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZAP70 (phospho Y493) antibody (ab194800)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, labeling ZAP70 (phospho Y493) with ab194800 at 1/50 dilution.
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Western blot - Anti-ZAP70 (phospho Y493) antibody (ab194800)All lanes : Anti-ZAP70 (phospho Y493) antibody (ab194800) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : PMA/TPA-treated Jurkat cell lysate
Lane 3 : Anisomycin-treated Jurkat cell lysate
Lane 4 : ATP-treated Jurkat cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 70 kDa
Exposure time: 30 secondsJurkat cells were treated by PMA/TPA (200 nM) at 37°C for 10 minutes, treated by Anisomycin (5 ug/ml) at 37°C for 30 minutes after serum-starvation overnight, or treated by ATP(5 mM) at 30°C for 1 hour.
Blocking buffer: 3% BSA.
