Anti-GSK3 alpha (phospho S21) antibody (ab131112)
Key features and details
- Rabbit polyclonal to GSK3 alpha (phospho S21)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GSK3 alpha (phospho S21) antibody
See all GSK3 alpha primary antibodies -
Description
Rabbit polyclonal to GSK3 alpha (phospho S21) -
Host species
Rabbit -
Specificity
ab131112 detects GSK3 alpha only when phosphorylated at serine 21. It does not detect GSK3 alpha phosphorylated at serine 9. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phosphopeptide conjugated to KLH, surrounding the phosphorylation site of serine 21 (T-S-S(p)-F-A) derived from Human GSK3 alpha.
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Positive control
- HeLa cells; 293 cell extracts treated with serum; Human breast carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131112 was affinity-purified by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GSK3 alpha (phospho S21) antibody (ab131112)All lanes : Anti-GSK3 alpha (phospho S21) antibody (ab131112) at 1/500 dilution
Lane 1 : 293 cell extracts, untreated
Lane 2 : 293 cell extracts, treated with serum
Predicted band size: 51 kDa
Incubate membrane with diluted antibody in 5% non-fat milk, 1 x TBS, 0.1% Tween20 at 4°C overnight with shaking. -
Immunocytochemistry/ Immunofluorescence - Anti-GSK3 alpha (phospho S21) antibody (ab131112)Immunoflourescent analysis of methanol-fixed HeLa cells labelling GSK3 alpha (phospho S21) with ab131112 at 1/100 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 alpha (phospho S21) antibody (ab131112)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma cells labelling GSK3 alpha (phospho S21) with ab131112 at 1/50 dilution. The image on the right is treated with blocking peptide.
