Anti-GSK3 alpha (phospho S21) antibody (ab28808)
Key features and details
- Rabbit polyclonal to GSK3 alpha (phospho S21)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GSK3 alpha (phospho S21) antibody
See all GSK3 alpha primary antibodies -
Description
Rabbit polyclonal to GSK3 alpha (phospho S21) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human GSK3a around the phosphorylation site of Serine 21.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GSK3 alpha (phospho S21) antibody (ab28808)All lanes : Anti-GSK3 alpha (phospho S21) antibody (ab28808) at 1/1000 dilution
Lane 1 : Extracts from ovary cancer
cells.
Lane 2 : Extracts from ovary cancer
cells. with synthesised non phosphopeptide at 1 µg/ml
Lane 3 : Extracts from ovary cancer
cells. with synthesised phosphopeptide at 1 µg/ml
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSK3 alpha (phospho S21) antibody (ab28808)Immunohistochemical analysis of paraffin embedded breast carcinoma, using primary CDK2 (Phospho-Thr160) ab28808 at 1:100 dilution and Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide. -
Immunocytochemistry/ Immunofluorescence - Anti-GSK3 alpha (phospho S21) antibody (ab28808)ICC/IF image of ab28808 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28808, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
