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HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control (ab193968)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR4513-32-7] to Cdk4 - Loading Control
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control
    See all Cdk4 primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR4513-32-7] to Cdk4 - Loading Control

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, HeLa, MCF7, K562, and Ramos whole cell lysates. IHC-P: FFPE human breast adenocarcinoma.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control (ab193968)
    Western blot - HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control (ab193968)
    All lanes : HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control (ab193968) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : CDK4 knockout HAP1 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 34 kDa


    Exposure time: 30 seconds


    ab193968 was shown to react with Cdk4 in wild-type HAP1 cells in Western blot. Loss of signal was observed when CDK4 knockout sample was used. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab193968 overnight at 4°C at a 1 in 5000 dilution and ab184095 (Mouse Anti-GAPDH antibody [mAbcam 9484] - Alexa Fluor® 680) at a 1 in 1000 dilution. Blots were developed with Optiblot ECL reagent (ab133456) and imaged.

  • Western blot - HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control (ab193968)
    Western blot - HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control (ab193968)
    All lanes : HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control (ab193968) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 34 kDa


    Exposure time: 90 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab193968 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control (ab193968)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control (ab193968)

    IHC image of Cdk4 staining in a section of formalin-fixed paraffin-embedded human breast adenocarcinoma*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab193968 at a working dilution of 1 in 500. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and manual) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control (ab193968)
    HRP Anti-Cdk4 antibody [EPR4513-32-7] - Loading Control (ab193968)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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