Anti-SERCA1 ATPase antibody [3D1H6] (ab233647)
![Anti-SERCA1 ATPase antibody [3D1H6] (ab233647)](https://www.abcam.com/ps/products/233/ab233647/Images/ab233647-306942-46.jpg "Anti-SERCA1 ATPase antibody [3D1H6] (ab233647)")
Key features and details
- Mouse monoclonal [3D1H6] to SERCA1 ATPase
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-SERCA1 ATPase antibody [3D1H6]
See all SERCA1 ATPase primary antibodies -
Description
Mouse monoclonal [3D1H6] to SERCA1 ATPase -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human, African green monkey
Predicted to work with: Rat, Rabbit, Cow
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Immunogen
Recombinant fragment corresponding to Human SERCA1 ATPase aa 450-650. Expressed in E.coli.
Database link: O14983 -
Positive control
- WB: Recombinant human SERCA1 ATPase (aa 487-631) protein; SERCA1 ATPase (aa 487-631)-hIgGFc transfected HEK-293 lysate; C2C12, COS-7, HeLa, K562 and Jurkat cell lysates. Flow cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3D1H6 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-SERCA1 ATPase antibody [3D1H6] (ab233647)All lanes : Anti-SERCA1 ATPase antibody [3D1H6] (ab233647) at 1/500 dilution
Lane 1 : C2C12 (mouse myoblast cell line) cell lysate.
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate.
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate.
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate.
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate.
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![Flow Cytometry - Anti-SERCA1 ATPase antibody [3D1H6] (ab233647)](https://www.abcam.com/ps/products/233/ab233647/Images/ab233647-306943-47.jpg)
Flow Cytometry - Anti-SERCA1 ATPase antibody [3D1H6] (ab233647)Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells using ab233647 (green) at 1/200 dilution compared with a negetive control (red).
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![Western blot - Anti-SERCA1 ATPase antibody [3D1H6] (ab233647)](https://www.abcam.com/ps/products/233/ab233647/Images/ab233647-306941-45.jpg)
Western blot - Anti-SERCA1 ATPase antibody [3D1H6] (ab233647)All lanes : Anti-SERCA1 ATPase antibody [3D1H6] (ab233647) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 : SERCA1 ATPase (aa487-631)-hIgGFc transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
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![Western blot - Anti-SERCA1 ATPase antibody [3D1H6] (ab233647)](https://www.abcam.com/ps/products/233/ab233647/Images/ab233647-306940-647.jpg)
Western blot - Anti-SERCA1 ATPase antibody [3D1H6] (ab233647)Anti-SERCA1 ATPase antibody [3D1H6] (ab233647) at 1/500 dilution + Recombinant human SERCA1 ATPase (aa 487-631) protein.Expected MW is 42 kDa.
