Anti-SERCA1 ATPase antibody (ab105172)
Key features and details
- Rabbit polyclonal to SERCA1 ATPase
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SERCA1 ATPase antibody
See all SERCA1 ATPase primary antibodies -
Description
Rabbit polyclonal to SERCA1 ATPase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide corresponding to Human SERCA1 ATPase aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab124619) -
Positive control
- This antibody gave a positive signal in Human, Mouse and Rat Skeletal Muscle tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseRatHumanAll applications GorillaApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 110 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 110 kDa).Target
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Function
Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. -
Tissue specificity
Skeletal muscle, fast twitch muscle (type II) fibers. -
Involvement in disease
Brody myopathy -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. -
Developmental stage
Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle. -
Cellular localization
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 487 Human
- Entrez Gene: 11937 Mouse
- Entrez Gene: 116601 Rat
- Omim: 108730 Human
- SwissProt: O14983 Human
- SwissProt: Q8R429 Mouse
- SwissProt: Q64578 Rat
- Unigene: 657344 Human
see all -
Alternative names
- fast twitch skeletal muscle isoform antibody
- AT2A1_HUMAN antibody
- ATP2A antibody
see all
Images
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Western blot - Anti-SERCA1 ATPase antibody (ab105172)All lanes : Anti-SERCA1 ATPase antibody (ab105172) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 99 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
The predicted molecular weight of SERCA1 ATPase is 110 kDa (SwissProt), however we expect to observe a banding pattern at 99 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Datasheets and documents
References (1)
ab105172 has been referenced in 1 publication.
- Oliver SR et al. Turning down the heat: Down-regulation of sarcolipin in a hibernating mammal. Neurosci Lett 696:13-19 (2018). PubMed: 30528880
Images
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Western blot - Anti-SERCA1 ATPase antibody (ab105172)All lanes : Anti-SERCA1 ATPase antibody (ab105172) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 99 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
The predicted molecular weight of SERCA1 ATPase is 110 kDa (SwissProt), however we expect to observe a banding pattern at 99 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
