Antibodies, Proteins, Kits and Reagents for Life Science

Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532)

Key features and details

Mouse monoclonal [CL2610] to GORASP2/GRASP55
Suitable for: ICC, IHC-P, WB
Reacts with: Human
Isotype: IgG2b

Product name

Anti-GORASP2/GRASP55 antibody [CL2610]
See all GORASP2/GRASP55 primary antibodies
Description

Mouse monoclonal [CL2610] to GORASP2/GRASP55
Host species

Mouse

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human GORASP2/GRASP55 aa 350 to the C-terminus.
Database link: Q9H8Y8

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Epitope

Binds to an epitope located within the peptide sequence DAASSLTVDVTPPTA (aa 405-412) as determined by overlapping synthetic peptides.
Positive control
- WB: U251 cell line lysate; IHC-P: Human prostate, epididymis, and endometrium tissues; ICC: HeLa, A431, MCF7, U2OS and U251 cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

CL2610
Isotype

IgG2b
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling GORASP2/GRASP55 with ab211532 at 1/5000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532)

All lanes : Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532)

Lane 1 : U-251 cells, transfected with control siRNA
Lane 2 : U-251 cells, transfected with target specific siRNA probe #1
Lane 3 : U-251 cells, transfected with target specific siRNA probe #2

Predicted band size: 47 kDa

Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.
Immunocytochemistry - Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532)

Immunofluorescent analysis of U251 cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab211532 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human epidiymis tissue labelling GORASP2/GRASP55 with ab211532 at 1/5000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling GORASP2/GRASP55 with ab211532 at 1/5000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling GORASP2/GRASP55 with ab211532 at 1/5000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532)

Immunofluorescent analysis of U2OS cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab211532 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.
Immunocytochemistry - Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532)

Immunofluorescent analysis of MCF7 cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab211532 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.
Immunocytochemistry - Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532)

Immunofluorescent analysis of A431 cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab211532 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.
Immunocytochemistry - Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532)

Immunofluorescent analysis of HeLa cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab211532 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.

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