Anti-GORASP2/GRASP55 antibody - C-terminal (ab192910)
Key features and details
- Rabbit polyclonal to GORASP2/GRASP55 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GORASP2/GRASP55 antibody - C-terminal
See all GORASP2/GRASP55 primary antibodies -
Description
Rabbit polyclonal to GORASP2/GRASP55 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human GORASP2/GRASP55 aa 400 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_056345.3).
Database link: Q9H8Y8 -
Positive control
- HeLa and 293T whole cell lysates.
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General notes
Protein previously labeled as GORASP2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab192910 was affinity purified using an epitope specific to GORASP2/GRASP55 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GORASP2/GRASP55 antibody - C-terminal (ab192910)All lanes : Anti-GORASP2/GRASP55 antibody - C-terminal (ab192910) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-GORASP2/GRASP55 antibody - C-terminal (ab192910)Detection of GORASP2/GRASP55 in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab192910 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
