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Anti-GORASP2/GRASP55 antibody - C-terminal (ab192910)

Key features and details

  • Rabbit polyclonal to GORASP2/GRASP55 - C-terminal
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-GORASP2/GRASP55 antibody - C-terminal
    See all GORASP2/GRASP55 primary antibodies

  • Description

    Rabbit polyclonal to GORASP2/GRASP55 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GORASP2/GRASP55 aa 400 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_056345.3).
    Database link: Q9H8Y8

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HeLa and 293T whole cell lysates.

  • General notes

    Protein previously labeled as GORASP2.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab192910 was affinity purified using an epitope specific to GORASP2/GRASP55 immobilized on solid support.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GORASP2/GRASP55 antibody - C-terminal (ab192910)
    Western blot - Anti-GORASP2/GRASP55 antibody - C-terminal (ab192910)
    All lanes : Anti-GORASP2/GRASP55 antibody - C-terminal (ab192910) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 47 kDa


    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer.

  • Immunoprecipitation - Anti-GORASP2/GRASP55 antibody - C-terminal (ab192910)
    Immunoprecipitation - Anti-GORASP2/GRASP55 antibody - C-terminal (ab192910)

    Detection of GORASP2/GRASP55 in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab192910 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

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