Anti-SA1 antibody - C-terminal (ab228758)
Key features and details
- Rabbit polyclonal to SA1 - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SA1 antibody - C-terminal
See all SA1 primary antibodies -
Description
Rabbit polyclonal to SA1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment within Human SA1 (C terminal). The exact sequence is proprietary.
Database link: Q8WVM7 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysate. IP: HeLa whole cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SA1 antibody - C-terminal (ab228758)All lanes : Anti-SA1 antibody - C-terminal (ab228758) at 1/3000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : SA1 shRNA transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 144 kDa5% SDS-PAGE
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Immunoprecipitation - Anti-SA1 antibody - C-terminal (ab228758)SA1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 μg ab228758. Western blot was performed from the immunoprecipitate using ab228758. Anti-rabbit IgG was used as secondary antibody.
Lane 1: HeLa whole cell extract (Input).
Lane 2: IgG instead of ab228758 in HeLa whole cell extract.
Lane 3: ab228758 IP in HeLa whole cell extract.
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Western blot - Anti-SA1 antibody - C-terminal (ab228758)All lanes : Anti-SA1 antibody - C-terminal (ab228758) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 144 kDa5% SDS-PAGE
