Anti-GORASP2/GRASP55 antibody (ab68713)
Key features and details
- Mouse polyclonal to GORASP2/GRASP55
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GORASP2/GRASP55 antibody
See all GORASP2/GRASP55 primary antibodies -
Description
Mouse polyclonal to GORASP2/GRASP55 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Full length protein corresponding to Human GORASP2/GRASP55. (NP_056345.3, 1 a.a. ~ 452 a.a)
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Positive control
- mouse neural stem cell lysate, GORASP2/GRASP55 transfected 293T lysate.
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General notes
Protein previously labeled as GORASP2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GORASP2/GRASP55 antibody (ab68713)All lanes : Anti-GORASP2/GRASP55 antibody (ab68713) at 1/500 dilution
Lane 1 : GORASP2 / GRASP5 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 48 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
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Western blot - Anti-GORASP2/GRASP55 antibody (ab68713) This image is courtesy of an abreview submitted by Melina Reisenberg, King's College London, United KingdomAnti-GORASP2/GRASP55 antibody (ab68713) at 1/500 dilution + neural stem cell lysate at 30 µg
Secondary
Peroxidase labeled horse anti-mouse IgG (H+L) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
