Antibodies, Proteins, Kits and Reagents for Life Science

412 099 ₸ Product size

7 ml 412 099 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CD276 antibody [SP206], prediluted (ab228178)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP206] to CD276, prediluted
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Human

Product name

Anti-CD276 antibody [SP206], prediluted
See all CD276 primary antibodies
Description

Rabbit monoclonal [SP206] to CD276, prediluted
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human tonsil, testis, stomach adenocarcinoma, lung, lung adenocarcinoma, lung SCC, colon, colon adenocarcinoma, breast, breast ductal carcinoma and stomach tissue. WB: HeLa, HEK293T and LNCaP cell lysates.
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP206
Isotype

IgG
Research areas

Western blot - Anti-CD276 antibody [SP206], prediluted (ab228178)

All lanes : Anti-CD276 antibody [SP206] (ab227670) at 1/400 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CD276 knockout HeLa cell lysate
Lane 3 : LNCaP cell lysate
Lane 4 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Observed band size: 90-110 kDa
why is the actual band size different from the predicted?

This data was developed using the same antibody clone in a different buffer formulation (ab227670).

Lanes 1- 4: Merged signal (red and green). Green - ab227670 observed at 90-110 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab227670 was shown to react with CD276 antigen in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261756 (knockout cell lysate ab257098) was used. Wild-type HeLa and CD276 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab227670 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Western blot - Anti-CD276 antibody [SP206], prediluted (ab228178)

All lanes : Anti-CD276 antibody [SP206] (ab227670) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : CD276 knockout HEK293T cell lysate
Lane 3 : LNCaP cell lysate
Lane 4 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Observed band size: 90-110 kDa why is the actual band size different from the predicted?

This data was developed using the same antibody clone in a different buffer formulation (ab227670).

Lanes 1-4: Merged signal (red and green). Green - ab227670 observed at 90-110 kDa. Red - loading control ab8245 observed at 36 kDa.

ab227670 Anti-CD276 antibody [SP206] was shown to specifically react with CD276 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266658 (knockout cell lysate ab257097) was used. Wild-type and CD276 knockout samples were subjected to SDS-PAGE. ab227670 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human stomach tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human breast tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human colon tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human lung SCC tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human lung tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human testis tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD276 with ab228178 in immunohistochemical analysis.
Anti-CD276 antibody [SP206], prediluted (ab228178)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com