Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6617] to FKBP51 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, IP, WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-FKBP51 antibody [EPR6617] - BSA and Azide free
    See all FKBP51 primary antibodies

  • Description

    Rabbit monoclonal [EPR6617] to FKBP51 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, IP, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human FKBP51 aa 400-500 (C terminal). The exact sequence is proprietary.
    Database link: Q13451

  • Positive control

    • IHC-P: Human colon, Rat stomach and Human prostatic hyperplasia tissue. ICC/IF: Jurkat cells. Flow Cyt: HeLa cells. IP: Jurkat cells.

  • General notes

    Ab242392 is the carrier-free version of ab126715. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab242392 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)
    Western blot - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: FKBP51 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab126715 observed at 51 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab126715 was shown to recognize FKBP51 in wild-type cells as signal was lost at the expected MW in FKBP51 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP51 knockout samples were subjected to SDS-PAGE. ab126715 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1,000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126715).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling FKBP51 with purified ab126715 at 1:250 dilution (1.156 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunoprecipitation - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)
    Immunoprecipitation - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)

    ab126715 (purified) at 1:20 dilution (2µg) immunoprecipitating FKBP51 in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab126715 & Jurkat whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126715 in Jurkat whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostatic hyperplasia tissue sections labeling FKBP51 with purified ab126715 at 1:250 dilution (1.156 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)
    Immunocytochemistry/ Immunofluorescence - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)
    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling FKBP51 with purified ab126715 at 1:100 dilution (2.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)
    Flow Cytometry - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FKBP51 with purified ab126715 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)

    ab126715, at 1/50 dilution, stains FKBP51 in paraffin embedded human colon tissue by immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126715).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)
    OI-RD Scanning - Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126715).

  • Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)
    Anti-FKBP51 antibody [EPR6617] - BSA and Azide free (ab242392)

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