Anti-FKBP51 antibody [Hi51B] (ab79844)
![Anti-FKBP51 antibody [Hi51B] (ab79844)](https://www.abcam.com/ps/products/79/ab79844/Images/ab79844-95257-anti-fkbp51-antibody-hi51b-immunofluorescence.jpg "Anti-FKBP51 antibody [Hi51B] (ab79844)")
Key features and details
- Mouse monoclonal [Hi51B] to FKBP51
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FKBP51 antibody [Hi51B]
See all FKBP51 primary antibodies -
Description
Mouse monoclonal [Hi51B] to FKBP51 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseWB Human
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Immunogen
Full length Human FKBP51, binding epitope in TPR domain
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Positive control
- WB: HeLa whole cell lysate (ab150035), rat kidney and rat and mouse testes tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
Hi51B -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-FKBP51 antibody [Hi51B] (ab79844)
ab79844 at 1/100 dilution staining FKBP51 in normal mouse MK cells (IF localization shown in red) by Immunocytochemistry/ immunofluorescence.
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![Western blot - Anti-FKBP51 antibody [Hi51B] (ab79844)](https://www.abcam.com/ps/products/79/ab79844/Images/ab79844-295055-anti-fkbp51-antibody-hi51b-western-blot.jpg)
Western blot - Anti-FKBP51 antibody [Hi51B] (ab79844)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: FKBP51 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab79844 observed at 51 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab79844 was shown to recognize FKBP51 in wild-type HAP1 cells as signal was lost at the expected MW in FKBP51 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP51 knockout samples were subjected to SDS-PAGE. ab79844 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
