Anti-beta II Tubulin antibody [EPR16773] (ab179512) at 1/10000 dilution + Human fetal brain lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Beta II Tubulin is highly expressed in brain tissues and cell lines derived from the central nervous system.

All lanes : Anti-beta II Tubulin antibody [EPR16773] (ab179512) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Beta II Tubulin is highly expressed in brain tissues and cell lines derived from the central nervous system.

All lanes : Anti-beta II Tubulin antibody [EPR16773] (ab179512) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat kidney lysate
Lane 8 : C6 (Rat glial tumor cells) whole cell lysate
Lane 9 : PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Beta II Tubulin is highly expressed in brain tissues and cell lines derived from the central nervous system.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling beta II Tubulin with ab179512 at 1/100 dilution, followed by anti-rabbit Alexa Fluor® 488 (ab150077) secondary antibody at 1/400 dilution (green). Confocal image shows both cytoplasm and nuclear staining on the SH-SY5Y cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: - ab179512 at 1/100 dilution followed by anti-mouse AlexaFluor® 594 (ab150120) at 1/500 dilution.
-ve control 2:. - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by anti-rabbit Alexa Fluor® 488 (ab150077) at 1/400 dilution.

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling beta II Tubulin with ab179512 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. High level of cytoplasmic and nuclear expression on neuron cells of Human cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemical analysis of paraffin-embedded Human glioma labeling beta II Tubulin with ab179512 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasmic staining on Human glioma cells is observed. Counter stained with Hematoxylin.

Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling beta II Tubulin with ab179512 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. High level of expression on neuron cells of Mouse cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling beta II Tubulin with ab179512 at 1/250 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. High level of expression on neuron cells of Rat cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling beta II Tubulin with ab179512 at 1/450 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Beta II Tubulin was immunoprecipitated from 1mg of Human fetal brain extract with ab179512 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab179512 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1: Human fetal brain extract. Lane 2: PBS instead of extract.

Blocking/Dilution buffer: 5% NFDM/TBST.