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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules Tubulin

Anti-beta II Tubulin antibody (ab151318)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-beta II Tubulin antibody (ab151318)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to beta II Tubulin
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-beta II Tubulin antibody
    See all beta II Tubulin primary antibodies
  • Description

    Rabbit polyclonal to beta II Tubulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis
  • Immunogen

    A recombinant protein fragment corresponding to a region within amino acids 243 and 445 of Human beta II Tubulin (UniProt ID: P68371).

  • Positive control

    • A431; NIH 3T3; Jurkat; 293T and Raji cell lysates; A431 cells
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Isotype/Loading Controls
    • Loading Controls
    • Tubulin
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin

Images

  • Western blot - Anti-beta II Tubulin antibody (ab151318)
    Western blot - Anti-beta II Tubulin antibody (ab151318)
    Anti-beta II Tubulin antibody (ab151318) at 1/10000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta II Tubulin antibody (ab151318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta II Tubulin antibody (ab151318)

    Paraffin-embedded human breast cancer tissue stained beta II tubuin using ab151318 at 1/250 dilution.

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

  • Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody (ab151318)
    Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody (ab151318)

    Immunofluorescence analysis of 4% paraformaldehyde fixed (at RT for 15 min) HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling beta II tubulin at cytoskeleton (green) using ab151318 at 1/500 dilution. Blue: Fluoroshield with DAPI. Scale bar= 10 μm. 

  • Western blot - Anti-beta II Tubulin antibody (ab151318)
    Western blot - Anti-beta II Tubulin antibody (ab151318)
    All lanes : Anti-beta II Tubulin antibody (ab151318) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 50 kDa



    10% SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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