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Neuroscience Neurotransmission Intracellular Signaling Kinases

Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4582] to TRPM7 + TRPM6
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-TRPM7 + TRPM6 antibody [EPR4582]
  • Description

    Rabbit monoclonal [EPR4582] to TRPM7 + TRPM6
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 86% homology with TRPM6 (ten amino acid stretch with 100% homology). ab109438 has been shown to cross react with TRPM6 in WB.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HuT-78, JAR, and HepG2 whole cell lysate (ab7900). HeLa cells and cell lysates.
  • General notes

    We have preliminary internal testing data to indicate this antibody may not react with mouse and rat species. Please contact us for more information

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4582
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Amyotrophic lateral sclerosis
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • More Ion Channels
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals

Images

  • Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)
    Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)
    All lanes : Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : HuT-78 cell lysate
    Lane 3 : JAR cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 213 kDa

  • Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)
    Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)
    All lanes : Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438) at 1/500 dilution

    Lane 1 : His-Tagged human TRPM6 (aa 1755 to 2022) recombinant protein with 5% NFDM/TBST
    Lane 2 : His-Tagged human TRPM7 (aa 1599 to 1986) recombinant protein with 5% NFDM/TBST

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 213 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds
  • Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)
    Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)
    Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438) at 1/1000 dilution (purified) + JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 213 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)
    Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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