Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-FKBP12 antibody [EPR3888] (ab92459)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3888] to FKBP12
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-FKBP12 antibody [EPR3888]
See all FKBP12 primary antibodies
Description

Rabbit monoclonal [EPR3888] to FKBP12
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human FKBP12 aa 50-150 (C terminal). The exact sequence is proprietary.
Positive control
- U937 and SH-SY5Y cell lysates. Fetal brain, human brain lysate, mouse heart lysate and rat heart tissue lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3888
Isotype

IgG
Research areas

Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: FKBP12 knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lane 4: Human brain whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab92459 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab92459 was shown to specifically react with FKBP12 in wild-type HAP1 cells as signal was lost in FKBP12 knockout cells. Wild-type and FKBP12 knockout samples were subjected to SDS-PAGE. Ab92459 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)

All lanes : Anti-FKBP12 antibody [EPR3888] (ab92459) at 1/2000 dilution

Lane 1 : Human brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat heart lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 12 kDa
Observed band size: 12 kDa

Blocking Buffer and concentration: 5% NFDM/TBST
Western blot - Anti-FKBP12 antibody [EPR3888] (ab92459)

All lanes : Anti-FKBP12 antibody [EPR3888] (ab92459) at 1/10000 dilution

Lane 1 : Fetal brain lysate
Lane 2 : U937 cell lysate
Lane 3 : SH-SY5Y cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 12 kDa
Anti-FKBP12 antibody [EPR3888] (ab92459)

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